A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder.

ABSTRACT: Because of the serious adulteration of goat milk, the rapid on-site detection of goat milk powder adulteration is needed. In this study, the CRISPR/Cas12a detection system combined with recombinase polymerase amplification (RPA) was employed to qualitatively detect the adulteration of goat milk powder with cattle-derived components. Specific primers and crRNA were designed and screened. After the optimization of RPA and the Cas system, the RPA-CRISPR/Cas12a detection method was established. The detection can complete the rapid identification of cattle-derived components in 45 min, without the assistant of large equipment. The absolute detectability of the RPA-CRISPR/Cas12a assay could reach 10^-2 ng/μL for cattle genomic DNA, and 1% (w/w) for cattle milk powder, which is s

SUBMITTER: Huang S 

PROVIDER: S-EPMC10137891 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature