Experimental rhinovirus infection induces an antiviral response in circulating B cells which is dysregulated in patients with asthma.

ABSTRACT:

Background

Rhinoviruses are the predominant cause of respiratory viral infections and are strongly associated with asthma exacerbations. While humoral immunity plays an important role during virus infections, cellular aspects of this response are less well understood. Here, we investigated the antiviral response of circulating B cells upon experimental rhinovirus infection in healthy individuals and asthma patients.

Methods

We purified B cells from experimentally infected healthy individuals and patients with asthma and subjected them to total RNA-sequencing. Rhinovirus-derived RNA was measured in isolated B cells using a highly sensitive PCR. B cells were stimulated with rhinovirus in vitro to further study gene expression, expression of antiviral proteins and B-cell differ

SUBMITTER: Wirz OF 

PROVIDER: S-EPMC10138744 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature