The viral packaging motor potentiates Kaposi's sarcoma-associated herpesvirus gene expression late in infection.

ABSTRACT: β- and γ-herpesviruses transcribe their late genes in a manner distinct from host transcription. This process is directed by a complex of viral transcriptional activator proteins that hijack cellular RNA polymerase II and an unknown set of additional factors. We employed proximity labeling coupled with mass spectrometry, followed by CRISPR and siRNA screening to identify proteins functionally associated with the Kaposi's sarcoma-associated herpesvirus (KSHV) late gene transcriptional complex. These data revealed that the catalytic subunit of the viral DNA packaging motor, ORF29, is both dynamically associated with the viral transcriptional activator complex and potentiates gene expression late in infection. Through genetic mutation and deletion of ORF29, we establish that its catalytic act

SUBMITTER: McCollum CO 

PROVIDER: S-EPMC10138851 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature