Reorganization and Suppression of Store-Operated Calcium Entry in Podocytes of Type 2 Diabetic Rats.

ABSTRACT: Type 2 diabetes mellitus (DM2) is a widespread metabolic disorder that results in podocyte damage and diabetic nephropathy. Previous studies demonstrated that TRPC6 channels play a pivotal role in podocyte function and their dysregulation is associated with development of different kidney diseases including nephropathy. Here, using single channel patch clamp technique, we demonstrated that non-selective cationic TRPC6 channels are sensitive to the Ca²⁺ store depletion in human podocyte cell line Ab8/13 and in freshly isolated rat glomerular podocytes. Ca²⁺ imaging indicated the involvement of ORAI and sodium-calcium exchanger in Ca²⁺ entry induced upon store depletion. In male rats fed a high-fat diet combined with a low-dose streptozotocin injection, which

SUBMITTER: Gusev K 

PROVIDER: S-EPMC10139047 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature