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Optogenetic activation of UCP1-dependent thermogenesis in brown adipocytes.

ABSTRACT: Brown adipocytes are unique in that they expend energy and produce heat to maintain euthermia through expression of uncoupling protein-1 (UCP1). Given their propensity to stimulate weight loss and promote resistance to obesity, they are a compelling interventional target for obesity-related disorders. Here, we tested whether an optogenetic approach could be used to activate UCP1-dependent thermogenesis in brown adipocytes. We generated brown adipocytes expressing a bacterial-derived photoactivatable adenylyl cyclase (bPAC) that, upon blue light stimulation, increases UCP1 expression, fuel uptake and thermogenesis. This unique system allows for precise, chemical free, temporal control of UCP1-dependent thermogenesis, which can aid in our understanding of brown adipocyte biology and development of therapies that target obesity-related disorders.

SUBMITTER: Doucette CC

PROVIDER: S-EPMC10139976 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Optogenetic activation of UCP1-dependent thermogenesis in brown adipocytes.

Doucette Chad C CC Nguyen Daniel C DC Barteselli Davide D Blanchard Sophia S Pelletier Masen M Kesharwani Devesh D Jachimowicz Ed E Su Su S Karolak Michele M Brown Aaron C AC

iScience 20230401 4

Brown adipocytes are unique in that they expend energy and produce heat to maintain euthermia through expression of uncoupling protein-1 (UCP1). Given their propensity to stimulate weight loss and promote resistance to obesity, they are a compelling interventional target for obesity-related disorders. Here, we tested whether an optogenetic approach could be used to activate UCP1-dependent thermogenesis in brown adipocytes. We generated brown adipocytes expressing a bacterial-derived photoactivat ...[more]

PMID: 37123235

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CPEB2-dependent translation of long 3'-UTR Ucp1 mRNA promotes thermogenesis in brown adipose tissue

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UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity.

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| S-EPMC2894971 | biostudies-literature

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