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Protocol to image deuterated propofol in living rat neurons using multimodal stimulated Raman scattering microscopy.

ABSTRACT: Propofol is a widely used anesthetic important in clinics, but like many other bioactive molecules, it is too small to be tagged and visualized by fluorescent dyes. Here, we present a protocol to visualize deuterated propofol in living rat neurons using stimulated Raman scattering (SRS) microscopy with carbon-deuterium bonds serving as a Raman tag. We describe the preparation and culture of rat neurons, followed by optimization of the SRS system. We then detail neuron loading and real-time imaging of anesthesia dynamics. For complete details on the use and execution of this protocol, please refer to Oda et al.¹.

SUBMITTER: Zhong W

PROVIDER: S-EPMC10140144 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Protocol to image deuterated propofol in living rat neurons using multimodal stimulated Raman scattering microscopy.

Zhong Wenying W Oda Robert R Ozeki Yasuyuki Y Yasui Masato M Nuriya Mutsuo M

STAR protocols 20230413 2

Propofol is a widely used anesthetic important in clinics, but like many other bioactive molecules, it is too small to be tagged and visualized by fluorescent dyes. Here, we present a protocol to visualize deuterated propofol in living rat neurons using stimulated Raman scattering (SRS) microscopy with carbon-deuterium bonds serving as a Raman tag. We describe the preparation and culture of rat neurons, followed by optimization of the SRS system. We then detail neuron loading and real-time imagi ...[more]

PMID: 37060560

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