Engineering resistance against Cucumber mosaic virus in Nicotiana tabacum through virus derived transgene expressing hairpin RNA.

ABSTRACT: Cucumber mosaic virus (CMV) is the one of notorious virus known for its ubiquitous nature and causes substantial yield loss worldwide. The resistance against the Cucumber mosaic virus (CMV) was envisaged in Nicotiana tabacum transgenic lines by introducing viral gene fragments. The chimeric hairpin RNA constructs incorporating 401 bp of coat protein, 411 bp of replicase protein and 361 bp of 2b gene were developed respectively and transformed into N. tabacum. The regenerated transgenic lines introduced with inverted repeats of CMV gene fragments exhibited enhanced resistance against CMV. The preliminary molecular screening and qPCR confirmed the integration of transgene in the transgenic lines. The spectrum of resistance in transgenic lines was evaluated by challenge i

SUBMITTER: Vinodhini J 

PROVIDER: S-EPMC10140202 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature