In-silico clearing approach for deep refractive index tomography by partial reconstruction and wave-backpropagation.

ABSTRACT: Refractive index (RI) is considered to be a fundamental physical and biophysical parameter in biological imaging, as it governs light-matter interactions and light propagation while reflecting cellular properties. RI tomography enables volumetric visualization of RI distribution, allowing biologically relevant analysis of a sample. However, multiple scattering (MS) and sample-induced aberration (SIA) caused by the inhomogeneity in RI distribution of a thick sample make its visualization challenging. This paper proposes a deep RI tomographic approach to overcome MS and SIA and allow the enhanced reconstruction of thick samples compared to that enabled by conventional linear-model-based RI tomography. The proposed approach consists of partial RI reconstruction using multiple holograms acquir

SUBMITTER: Yasuhiko O 

PROVIDER: S-EPMC10140380 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature