Deciphering mechanically activated ion channels at the single-channel level in dorsal root ganglion neurons.

ABSTRACT: Mechanically activated (MA) ion channels confer somatosensory neurons with the ability to sense a wide range of mechanical stimuli. MA ion channel activity in somatosensory neurons is best described by the electrophysiological recordings of MA currents in cultured dorsal root ganglion (DRG) neurons. Biophysical and pharmacological characterization of DRG MA currents has guided the field in screening/confirming channel candidates that induce the currents and facilitate the mechanosensory response. But studies on DRG MA currents have relied mostly on whole-cell macroscopic current properties obtained by membrane indentation, and little is known about the underlying MA ion channels at the single-channel level. Here, by acquiring indentation-induced macroscopic currents as well as stretch-acti

SUBMITTER: Murthy SE 

PROVIDER: S-EPMC10140383 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature