SynapseCLR: Uncovering features of synapses in primary visual cortex through contrastive representation learning.

ABSTRACT: 3D electron microscopy (EM) connectomics image volumes are surpassing 1 mm³, providing information-dense, multi-scale visualizations of brain circuitry and necessitating scalable analysis techniques. We present SynapseCLR, a self-supervised contrastive learning method for 3D EM data, and use it to extract features of synapses from mouse visual cortex. SynapseCLR feature representations separate synapses by appearance and functionally important structural annotations. We demonstrate SynapseCLR's utility for valuable downstream tasks, including one-shot identification of defective synapse segmentations, dataset-wide similarity-based querying, and accurate imputation of annotations for unlabeled synapses, using manual annotation of only 0.2% of the dataset's synapses. In particular

SUBMITTER: Wilson A 

PROVIDER: S-EPMC10140600 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature