Enteroendocrine Cells Protect the Stem Cell Niche by Regulating Crypt Metabolism in Response to Nutrients.

ABSTRACT:

Background & aims

The intestinal stem cell niche is exquisitely sensitive to changes in diet, with high-fat diet, caloric restriction, and fasting resulting in altered crypt metabolism and intestinal stem cell function. Unlike cells on the villus, cells in the crypt are not immediately exposed to the dynamically changing contents of the lumen. We hypothesized that enteroendocrine cells (EECs), which sense environmental cues and in response release hormones and metabolites, are essential for relaying the luminal and nutritional status of the animal to cells deep in the crypt.

Methods

We used the tamoxifen-inducible VillinCreERT2 mouse model to deplete EECs (Neurog3^fl/fl) from adult intestinal epithelium and we generated human intestinal organoids from wild-type and

SUBMITTER: McCauley HA 

PROVIDER: S-EPMC10140799 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature