Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans.

ABSTRACT: Toad venom is a traditional Chinese medicine with high medicinal value. The existing quality evaluation standards of toad venom have obvious limitations because of the lack of research on proteins. Thus, it is necessary to screen suitable quality markers and establish appropriate quality evaluation methods for toad venom proteins to guarantee their safety and efficacy in clinical applications. SDS-PAGE, HPLC, and cytotoxicity assays were used to analyze differences in protein components of toad venom from different areas. Functional proteins were screened as potential quality markers by proteomic and bioinformatic analyses. The protein components and small molecular components of toad venom were not correlated in content. Additionally, the protein component had strong cytotoxicity. Proteom

SUBMITTER: Yang M 

PROVIDER: S-EPMC10141085 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature