Evaluating Novel Quantification Methods for Infectious Baculoviruses.

ABSTRACT: Accurate and rapid quantification of (infectious) virus titers is of paramount importance in the manufacture of viral vectors and vaccines. Reliable quantification data allow efficient process development at a laboratory scale and thorough process monitoring in later production. However, current gold standard applications, such as endpoint dilution assays, are cumbersome and do not provide true process analytical monitoring. Accordingly, flow cytometry and quantitative polymerase chain reaction have attracted increasing interest in recent years, offering various advantages for rapid quantification. Here, we compared different approaches for the assessment of infectious viruses, using a model baculovirus. Firstly, infectivity was estimated by the quantification of viral nucleic acids in inf

SUBMITTER: Lothert K 

PROVIDER: S-EPMC10141099 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature