Optical Monitoring of In Situ Iron Loading into Single, Native Ferritin Proteins.

ABSTRACT: Ferritin is a protein that stores and releases iron to prevent diseases associated with iron dysregulation in plants, animals, and bacteria. The conversion between iron-loaded holo-ferritin and empty apo-ferritin is an important process for iron regulation. To date, studies of ferritin have used either ensemble measurements to quantify the characteristics of a large number of proteins or single-molecule approaches to interrogate labeled or modified proteins. Here we demonstrate the first real-time study of the dynamics of iron ion loading and biomineralization within a single, unlabeled ferritin protein. Using optical nanotweezers, we trapped single apo- and holo-ferritins indefinitely, distinguished one from the other, and monitored their structural dynamics in real time. The study presen

SUBMITTER: Yousefi A 

PROVIDER: S-EPMC10141409 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature