Passive Recording of Bioelectrical Signals from Non-Excitable Cells by Fluorescent Mirroring.

ABSTRACT: Bioelectrical variations trigger different cell responses, including migration, mitosis, and mutation. At the tissue level, these actions result in phenomena such as wound healing, proliferation, and pathogenesis. Monitoring these mechanisms dynamically is highly desirable in diagnostics and drug testing. However, existing technologies are invasive: either they require physical access to the intracellular compartments, or they imply direct contact with the cellular medium. Here, we present a novel approach for the passive recording of electrical signals from non-excitable cells adhering to 3D microelectrodes, based on optical mirroring. Preliminary results yielded a fluorescence intensity output increase of the 5,8% in the presence of a HEK-293 cell on the electrode compared to bare microe

SUBMITTER: Moreddu R 

PROVIDER: S-EPMC10141418 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature