The Interplay Between Metabolites and MicroRNAs in Aqueous Humor to Coordinate Corneal Endothelium Integrity

ABSTRACT:

Purpose

The purpose of the study was to clarify the interplay between metabolites and microRNAs (miRs) in the aqueous humor (AqH) of bullous keratopathy (BK) patients to retain human corneal endothelium (HCE) integrity.

Design

Prospective, comparative, observational study.

Participants

A total of 55 patients with BK and 31 patients with cataract (Cat) as control.

Methods

A biostatic analysis of miRs and metabolites in the AqH, hierarchical clustering, and a least absolute shrinkage and selection operator (Lasso) analysis were employed. The miR levels in AqH of BK (n = 18) and Cat (n = 8) patients were determined using 3D-Gene human miR chips. Hierarchical clusters of metabolites detected by liquid chromatography–mass spectrometry or gas chromatography–ma

SUBMITTER: Ueno M 

PROVIDER: S-EPMC10141542 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature