The intratumour microbiota and neutrophilic inflammation in squamous cell vulvar carcinoma microenvironment.

ABSTRACT:

Background

A causal link between microbiota composition (dysbiosis) and oncogenesis has been demonstrated for several types of cancer. Neutrophils play a role in both immune protection against bacterial threats and carcinogenesis. This study aimed to characterise intratumoral bacteria in vulvar squamous cell carcinoma (VSCC) and their putative effect on neutrophil recruitment and cancer progression.

Methods

Clinical material was obtained from 89 patients with VSCC. Next-generation sequencing (NGS) of 16S rRNA and quantitative polymerase chain reaction (qPCR) were used to detect bacterial species in VSCC. To verify neutrophil activation, CD66b expression in tumour specimens was analysed by immunohistochemistry (IHC). Subsequently, IHC was applied to detect the main neutrophil

SUBMITTER: Rustetska N 

PROVIDER: S-EPMC10141905 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature