Evaluation of GC/MS-Based ¹³C-Positional Approaches for TMS Derivatives of Organic and Amino Acids and Application to Plant ¹³C-Labeled Experiments.

ABSTRACT: Analysis of plant metabolite ¹³C-enrichments with gas-chromatography mass spectrometry (GC/MS) has gained interest recently. By combining multiple fragments of a trimethylsilyl (TMS) derivative, ¹³C-positional enrichments can be calculated. However, this new approach may suffer from analytical biases depending on the fragments selected for calculation leading to significant errors in the final results. The goal of this study was to provide a framework for the validation of ¹³C-positional approaches and their application to plants based on some key metabolites (glycine, serine, glutamate, proline, α-alanine and malate). For this purpose, we used tailor-made ¹³C-PT standards, harboring known carbon isotopologue distributions and ¹³C-posi

SUBMITTER: Dellero Y 

PROVIDER: S-EPMC10142191 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature