Increasing temperature denatures canine IgG reducing its ability to inhibit heartworm antigen detection.

ABSTRACT:

Background

Immune complexing of target antigen to high affinity host antibody is recognized to impact the sensitivity of commercial heartworm antigen tests. Published information describing the effect of heat on interfering canine host antibodies is lacking. Immune complex dissociation (ICD) by heat treatment of serum for samples initially testing negative for heartworm antigen increases sensitivity of commercial antigen tests, particularly for single sex or low adult infection intensities. In this study the stability and nature of the targeted epitope and mechanism of heat ICD were examined.

Methods

Canine IgG was isolated using protein-A columns from serum originating from four dogs evaluated after necropsy: one dog with evidence of previously cleared infection and three d

SUBMITTER: Gruntmeir JM 

PROVIDER: S-EPMC10142223 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature