Novel organoid construction strategy for non-involuting congenital hemangioma for drug validation.

ABSTRACT:

Background

Non-involuting congenital hemangiomas (NICHs) are fully formed vascular tumors at birth with distinctive clinical, radiologic, and histopathological profiles. In the literature, there is no effective therapy strategy for patients with NICH except surgery. Currently, no cell line or animal model exists for studying the mechanism of NICH and drug validation. We plan to construct a new strategy by constructing NICH organoids for further study.

Result

Here, we report a novel NICH organoid system construction and optimization process. Both HE and immunohistological staining exactly matched NICH tissue. We further performed transcriptome analysis to elucidate the characteristics of NICH organoids. Both NICH tissue and NICH organoids manifested similar trends in download

SUBMITTER: Wei H 

PROVIDER: S-EPMC10142414 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature