An optimized approach for multiplexing single-nuclear ATAC-seq using oligonucleotide-conjugated antibodies.

ABSTRACT:

Background

Single-cell technologies to analyze transcription and chromatin structure have been widely used in many research areas to reveal the functions and molecular properties of cells at single-cell resolution. Sample multiplexing techniques are valuable when performing single-cell analysis, reducing technical variation and permitting cost efficiencies. Several commercially available methods have been used in many scRNA-seq studies. On the other hand, while several methods have been published, multiplexing techniques for single nuclear assay for transposase-accessible chromatin (snATAC)-seq assays remain under development. We developed a simple nucleus hashing method using oligonucleotide-conjugated antibodies recognizing nuclear pore complex proteins, NuHash, to perform snATAC

SUBMITTER: Bera BS 

PROVIDER: S-EPMC10142415 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature