Bispecific T cell-engager targeting oncofetal chondroitin sulfate induces complete tumor regression and protective immune memory in mice.

ABSTRACT:

Background

The malaria protein VAR2CSA binds oncofetal chondroitin sulfate (ofCS), a unique chondroitin sulfate, expressed on almost all mammalian cancer cells. Previously, we produced a bispecific construct targeting ofCS and human T cells based on VAR2CSA and anti-CD3 (V-aCD3^Hu). V-aCD3^Hu showed efficacy against xenografted tumors in immunocompromised mice injected with human immune cells at the tumor site. However, the complex effects potentially exerted by the immune system as a result of the treatment cannot occur in mice without an immune system. Here we investigate the efficacy of V-aCD3^Mu as a monotherapy and combined with immune checkpoint inhibitors in mice with a fully functional immune system.

Methods

We produced a bispecific cons

SUBMITTER: Skeltved N 

PROVIDER: S-EPMC10142489 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature