Establishment of a Simple and Rapid Nucleic Acid Detection Method for Hookworm Identification.

ABSTRACT: Hookworm infection is one of the most common neglected tropical diseases and is mainly found in tropical and subtropical areas. Two species of human hookworm are distributed in China, i.e., Ancylostoma duodenale (AD) and Necator americanus (NA).

Background

Traditional microscopic technology such as the Kato-Katz method is not suitable for hookworm diagnosis due to the rapid degeneration of fragile hookworm eggs or for species identification of hookworm infection. The aim of the present study was to establish and evaluate a novel nucleic acid detection method based on recombinase-aided isothermal amplification (RAA) for the detection of hookworm infections and species identification.

Methods

Based on the specific target gene sequences of hookworms (5.8S rRNA</

SUBMITTER: Ding X 

PROVIDER: S-EPMC10142964 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature