Extraction of High-Quality RNA from S. aureus Internalized by Endothelial Cells.

ABSTRACT: Staphylococcus aureus evades antibiotic therapy and antimicrobial defenses by entering human host cells. Bacterial transcriptomic analysis represents an invaluable tool to unravel the complex interplay between host and pathogen. Therefore, the extraction of high-quality RNA from intracellular S. aureus lays the foundation to acquire meaningful gene expression data. In this study, we present a novel and straightforward strategy to isolate RNA from internalized S. aureus after 90 min, 24 h, and 48 h postinfection. Real-time PCR data were obtained for the target genes agrA and fnba, which play major roles during infection. The commonly used reference genes gyrB, aroE, tmRNA, gmk, and hu were analyzed under different conditi

SUBMITTER: Maurer M 

PROVIDER: S-EPMC10143013 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature