Effect of Methionine on Gene Expression in Komagataella phaffii Cells.

ABSTRACT: Komagataella phaffii yeast plays a prominent role in modern biotechnology as a recombinant protein producer. For efficient use of this yeast, it is essential to study the effects of different media components on its growth and gene expression. We investigated the effect of methionine on gene expression in K. phaffii cells using RNA-seq analysis. Several gene groups exhibited altered expression when K. phaffii cells were cultured in a medium with methanol and methionine, compared to a medium without this amino acid. Methionine primarily affects the expression of genes involved in its biosynthesis, fatty acid metabolism, and methanol utilization. The AOX1 gene promoter, which is widely used for heterologous expression in K. phaffii, is downregulated in meth

SUBMITTER: Ianshina T 

PROVIDER: S-EPMC10143545 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature