A Real-Time PCR Approach for Rapid Detection of Viable Salmonella Enteritidis in Shell Eggs.

ABSTRACT: Rapid and robust detection assays for Salmonella Enteritidis (SE) in shell eggs are essential to enable a quick testing turnaround time (TAT) at the earliest checkpoint and to ensure effective food safety control. Real-time polymerase chain reaction (qPCR) assays provide a workaround for the protracted lead times associated with conventional Salmonella diagnostic testing. However, DNA-based analysis cannot reliably discriminate between signals from viable and dead bacteria. We developed a strategy based on an SE qPCR assay that can be integrated into system testing to accelerate the detection of viable SE in egg-enriched cultures and verify the yielded SE isolates. The specificity of the assay was evaluated against 89 Salmonella strains, and SE was accurately identifie

SUBMITTER: Chan SH 

PROVIDER: S-EPMC10143610 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature