Heterologous Display of Chlamydia trachomatis PmpD Passenger at the Surface of Salmonella OMVs.

ABSTRACT: Chlamydia trachomatis is the bacterial pathogen that causes most cases of sexually transmitted diseases annually. To combat the global spread of asymptomatic infection, development of effective (mucosal) vaccines that offer both systemic and local immune responses is considered a high priority. In this study, we explored the expression of C. trachomatis full-length (FL) PmpD, as well as truncated PmpD passenger constructs fused to a "display" autotransporter (AT) hemoglobin protease (HbpD) and studied their inclusion into outer membrane vesicles (OMVs) of Escherichia coli and Salmonella Typhimurium. OMVs are considered safe vaccine vectors well-suited for mucosal delivery. By using E. coli AT HbpD-fusions of chimeric constructs we improved surface display

SUBMITTER: Huynh DT 

PROVIDER: S-EPMC10145130 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature