Heterologous expression of MirMAN enhances root development and salt tolerance in Arabidopsis.

ABSTRACT:

Introduction

β-Mannanase is a plant cell wall remodeling enzyme involved in the breakdown of hemicellulose and plays an important role in growth by hydrolyzing the mannan-like polysaccharide, but its function in adaptation to salt stress has been less studied.

Methods

Based on cloned the mannanase (MAN) gene from Mirabilis jalapa L., the study was carried out by heterologously expressing the gene in Arabidopsis thaliana, and then observing the plant phenotypes and measuring relevant physiological and biochemical indicators under 150 mM salt treatment.

Results and discussion

The results indicate that MirMAN is a protein with a glycohydrolase-specific structural domain located in the cell wall. We first found that MirMAN reduced the susceptibility o

SUBMITTER: Xu J 

PROVIDER: S-EPMC10145921 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature