Alteration of m⁶A-Tagged RNA Profiles in Bone Originated from Periprosthetic Joint Infection.

ABSTRACT: Periprosthetic joint infection (PJI) is a devastating complication. This study aimed to unravel the veil of the N⁶-methyladenine (m⁶A) modification in PJI. Synovium, synovial fluid, sonication fluid and bone samples were collected intraoperatively from Staphylococcus aureus PJI and aseptic failure (AF) patients. The overall m⁶A level was detected by the m⁶A RNA methylation quantification kit, and the expression of m⁶A-related genes was quantified by real-time PCR and Western blot. Finally, an epitranscriptomic microarray and bioinformatics analysis were performed. We showed that there was a significant difference in overall m⁶A level between the PJI group and the AF group (PJI group had a higher overall m⁶A

SUBMITTER: Cai Y 

PROVIDER: S-EPMC10146075 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature