Characterization of Feruloyl Esterase from Klebsiella oxytoca Z28 and Its Application in the Release of Ferulic Acid from De-Starching Wheat Bran.

ABSTRACT: Feruloyl esterase (EC3.1.1.73; FAE) can degrade biomass to release ferulic acid (FA), which has a high application in bioprocessing, food, pharmaceutical, paper, feed, and other industrial fields. A strain of Klebsiella oxytoca Z28 with ferulic esterase activity was screened from Daqu. In addition, the FAE gene was expressed in Escherichia coli BL21 (DE3). The enzyme consists of 340 amino acids with a molecular mass of 37.7 kDa. The FAE enzyme activity was 463 U/L when the substrate was ethyl 4-hydroxy-3-methoxycinnamate and the optimum temperature and pH were 50 °C and 8.0, respectively. The enzyme had good stability at temperatures of 25-40 °C and a pH of 8.0. Ba²⁺, Cu²⁺, Mn²⁺, and Ca²⁺ had a strong inhibitory effect on the enzyme

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10146132 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature