Pollution-Free and Highly Sensitive Lactate Detection in Cell Culture Based on a Microfluidic Chip.

ABSTRACT: Cell metabolite detection is important for cell analysis. As a cellular metabolite, lactate and its detection play an important role in disease diagnosis, drug screening and clinical therapeutics. This paper reports a microfluidic chip integrated with a backflow prevention channel for cell culture and lactate detection. It can effectively realize the upstream and downstream separation of the culture chamber and the detection zone, and prevent the pollution of cells caused by the potential backflow of reagent and buffer solutions. Due to such a separation, it is possible to analyze the lactate concentration in the flow process without contamination of cells. With the information of residence time distribution of the microchannel networks and the detected time signal in the detection chamber

SUBMITTER: Shi J 

PROVIDER: S-EPMC10146269 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature