Exploring the Fate of Antibody-Encoding pDNA after Intramuscular Electroporation in Mice.

ABSTRACT: DNA-based antibody therapy seeks to administer the encoding nucleotide sequence rather than the antibody protein. To further improve the in vivo monoclonal antibody (mAb) expression, a better understanding of what happens after the administration of the encoding plasmid DNA (pDNA) is required. This study reports the quantitative evaluation and localization of the administered pDNA over time and its association with corresponding mRNA levels and systemic protein concentrations. pDNA encoding the murine anti-HER2 4D5 mAb was administered to BALB/c mice via intramuscular injection followed by electroporation. Muscle biopsies and blood samples were taken at different time points (up to 3 months). In muscle, pDNA levels decreased 90% between 24 h and one week post treatment (p < 0.0001).

SUBMITTER: Cuypers ML 

PROVIDER: S-EPMC10146361 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature