Joint Microbiota Activity and Dietary Assessment through Urinary Biomarkers by LC-MS/MS.

ABSTRACT: Accurate dietary assessment in nutritional research is a huge challenge, but essential. Due to the subjective nature of self-reporting methods, the development of analytical methods for food intake and microbiota biomarkers determination is needed. This work presents an ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) method for the quantification and semi quantification of 20 and 201 food intake biomarkers (BFIs), respectively, as well as 7 microbiota biomarkers applied to 208 urine samples from lactating mothers (M) (N = 59). Dietary intake was assessed through a 24 h dietary recall (R24h). BFI analysis identified three distinct clusters among samples: samples from clusters 1 and 3 presented higher concentrations of most biomarkers tha

SUBMITTER: Ramos-Garcia V 

PROVIDER: S-EPMC10146414 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature