Phenotypic screening using waveform analysis of synchronized calcium oscillations in primary cortical cultures.

ABSTRACT: At present, in vitro phenotypic screening methods are widely used for drug discovery. In the field of epilepsy research, measurements of neuronal activities have been utilized for predicting efficacy of anti-epileptic drugs. Fluorescence measurements of calcium oscillations in neurons are commonly used for measurement of neuronal activities, and some anti-epileptic drugs have been evaluated using this assay technique. However, changes in waveforms were not quantified in previous reports. Here, we have developed a high-throughput screening system containing a new analysis method for quantifying waveforms, and our method has successfully enabled simultaneous measurement of calcium oscillations in a 96-well plate. Features of waveforms were extracted automatically and allowed the characteriza

SUBMITTER: Sakaguchi R 

PROVIDER: S-EPMC10146483 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature