Mechanical strain treatment improves nuclear transfer reprogramming efficiency by enhancing chromatin accessibility.

ABSTRACT: Cellular mechanical properties are considered to be important factors affecting cell fate transitions, but the links between cellular mechanical properties and transition efficiency and chromatin structure remain elusive. Here, we predicted that mechanical strain treatment could induce signatures of cellular dedifferentiation and transdifferentiation, and we validated this prediction by showing that mechanical strain-treated mouse cumulus cells (CCs) exhibit significantly improved somatic cell nuclear transfer (SCNT) reprogramming efficiency. We found that the chromatin accessibility of CCs was globally increased by mechanical strain treatment and that this increase was partially mediated by the induction of the YAP-TEAD interaction. Moreover, using mechanical strain-treated CCs could prev

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10147550 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature