Assessment of a multisite standardized biospecimen collection protocol for immune phenotyping in neurodevelopmental disorders.

ABSTRACT: Multisite collection and preservation of peripheral blood mononuclear cells (PBMCs) for centralized analysis is an indispensable strategy for large cohort immune phenotyping studies. However, the absence of cross-site standardized protocols introduces unnecessary sample variance. Here we describe the protocol implemented by the Province of Ontario Neurodevelopmental Disorders (POND) Network's immune platform for the multisite collection, processing, and cryopreservation of PBMCs. We outline quality control standards and evaluate the performance of our PBMC processing and storage protocol. We also describe the Child Immune History Questionnaire results, an assessment tool evaluating pre-existing immune conditions in children with neurodevelopmental disorders (NDDs). Cell viability was asses

SUBMITTER: Cleary S 

PROVIDER: S-EPMC10147654 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature