OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems.

ABSTRACT: Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument named 'OptoRheo' that combines light sheet fluorescence microscopy with particle tracking microrheology. OptoRheo lets us image cells in 3D as they proliferate over several days while simultaneously sensing the mechanical properties of the surrounding extracellular and pericellular matrix at a sub-cellular length scale. OptoRheo can be used in two operational modalities (with and without an optical trap) to extend the dynamic range of microrheology measurements. We corroborated this by characterising the ECM surrounding live breast cancer cells

SUBMITTER: Mendonca T 

PROVIDER: S-EPMC10147656 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature