Differentiation of uniparental human embryonic stem cells into granulosa cells reveals a paternal contribution to gonadal development.

ABSTRACT: Genomic imprinting underlies the mammalian requirement for sexual reproduction. Nonetheless, the relative contribution of the two parental genomes during human development is not fully understood. Specifically, a fascinating question is whether the formation of the gonad, which holds the ability to reproduce, depends on equal contribution from both parental genomes. Here, we differentiated androgenetic and parthenogenetic human pluripotent stem cells (hPSCs) into ovarian granulosa-like cells (GLCs). We show that in contrast to biparental and androgenetic cells, parthenogenetic hPSCs present a reduced capacity to differentiate into GLCs. We further identify the paternally expressed gene IGF2 as the most upregulated imprinted gene upon differentiation. Remarkably, while IGF2 knockout androge

SUBMITTER: Keshet G 

PROVIDER: S-EPMC10147827 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature