Mechanical memory stored through epigenetic remodeling reduces cell therapeutic potential.

ABSTRACT: Understanding how cells remember previous mechanical environments to influence their fate, or mechanical memory, informs the design of biomaterials and therapies in medicine. Current regeneration therapies, such as cartilage regeneration procedures, require 2D cell expansion processes to achieve large cell populations critical for the repair of damaged tissues. However, the limit of mechanical priming for cartilage regeneration procedures before inducing long-term mechanical memory following expansion processes is unknown, and mechanisms defining how physical environments influence the therapeutic potential of cells remain poorly understood. Here, we identify a threshold to mechanical priming separating reversible and irreversible effects of mechanical memory. After 16 population doublings

SUBMITTER: Scott AK 

PROVIDER: S-EPMC10147835 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature