Utilizing Metagenomic Next-Generation Sequencing (mNGS) for Rapid Pathogen Identification and to Inform Clinical Decision-Making: Results from a Large Real-World Cohort.

ABSTRACT:

Introduction

Clinical metagenomic next-generation sequencing (mNGS) has proven to be a powerful diagnostic tool in pathogen detection. However, its clinical utility has not been thoroughly evaluated.

Methods

In this single-center prospective study at the First Affiliated Hospital of Soochow University, a total of 228 samples from 215 patients suspected of having acute or chronic infections between June 2018 and December 2018 were studied. Samples that met the mNGS quality control (QC) criteria (N = 201) were simultaneously analyzed using conventional tests (CTs), including multiple clinical microbiological tests and real-time PCR (if applicable).

Results

Pathogen detection results of mNGS in the 201 QC-passed samples were compared to CTs and exhibited a sensitivity of

SUBMITTER: Xu J 

PROVIDER: S-EPMC10147866 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature