Digital morphometry and cluster analysis identifies four types of melanocyte during uveal melanoma progression.

ABSTRACT:

Background

Several types of benign and malignant uveal melanocytes have been described based on their histological appearance. However, their characteristics have not been quantified, and their distribution during progression from normal choroidal melanocytes to primary tumors and metastases has not been reported.

Methods

A total of 1,245,411 digitally scanned melanocytes from normal choroid, choroidal nevi, primary uveal melanomas, and liver metastases were entered into two-step cluster analyses to delineate cell types based on measured morphometric characteristics and expression of protein markers.

Results

Here we show that a combination of the area and circularity of cell nuclei, and BAP-1 expression in nuclei and cytoplasms yields the highest silhouette of cohesio

SUBMITTER: Stalhammar G 

PROVIDER: S-EPMC10147908 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature