Quantitative analysis of autophagy reveals the role of ATG9 and ATG2 in autophagosome formation.

ABSTRACT: Autophagy is a catabolic pathway required for the recycling of cytoplasmic materials. To define the mechanisms underlying autophagy it is critical to quantitatively characterize the dynamic behavior of autophagy factors in living cells. Using a panel of cell lines expressing HaloTagged autophagy factors from their endogenous loci, we analyzed the abundance, single-molecule dynamics, and autophagosome association kinetics of autophagy proteins involved in autophagosome biogenesis. We demonstrate that autophagosome formation is inefficient and ATG2-mediated tethering to donor membranes is a key commitment step in autophagosome formation. Furthermore, our observations support the model that phagophores are initiated by the accumulation of autophagy factors on mobile ATG9 vesicles, and that th

SUBMITTER: Broadbent DG 

PROVIDER: S-EPMC10148237 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature