Proteomics identifies differentially expressed proteins in glioblastoma U87 cells treated with hederagenin.

ABSTRACT:

Objective

We investigated differentially expressed proteins (DEPs) in human glioblastoma U87 cells after treatment with hederagenin as a therapeutic screening mechanism and provided a theoretical basis for hederagenin in treating glioblastoma.

Methods

The Cell Counting Kit 8 assay was used to analyze the inhibitory effect of hederagenin on the proliferation of U87 cells. Protein was identified by tandem mass tags and LC-MS/MS analysis techniques. Annotation of DEPs, Gene Ontology enrichment and function, and Kyoto Encyclopedia of Genes and Genomes pathways and domains were all examined by bioinformatics. According to the TMT results, hub protein was selected from DEPs for WB verification.

Results

Protein quantitative analysis found 6522 proteins in total. Compared wit

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10148390 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature