Single-cell sequencing analysis fibrosis provides insights into the pathobiological cell types and cytokines of radiation-induced pulmonary fibrosis.

ABSTRACT:

Background

Radiotherapy is an essential treatment for chest cancer. Radiation-induced pulmonary fibrosis (RIPF) is an almost irreversible interstitial lung disease; however, its pathogenesis remains unclear.

Methods

We analyzed specific changes in cell populations and potential markers by using single-cell sequencing datasets from the Sequence Read Archive database, PERFORMED from control (0 Gy) and thoracic irradiated (20 Gy) mouse lungs at day 150 post-radiation. We performed IHC and ELISA on lung tissue and cells to validate the potential marker cytokines identified by the analysis on rat thoracic irradiated molds (30 Gy).

Results

Single-cell sequencing analysis showed changes in abundance across cell types and at the single-cell level, with B and T cells showing t

SUBMITTER: Sun Z 

PROVIDER: S-EPMC10148423 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature