Mechanism of Zn²⁺ regulation of cellulase production in Trichoderma reesei Rut-C30.

ABSTRACT:

Background

Trichoderma reesei Rut-C30 is a hypercellulolytic mutant strain that degrades abundant sources of lignocellulosic plant biomass, yielding renewable biofuels. Although Zn²⁺ is an activator of enzymes in almost all organisms, its effects on cellulase activity in T. reesei have yet to be reported.

Results

Although high concentrations of Zn²⁺ severely suppressed the extension of T. reesei mycelia, the application of 1-4 mM Zn²⁺ enhanced cellulase and xylanase production in the high-yielding cellulase-producing Rut-C30 strain of T. reesei. Expression of the major cellulase, xylanase, and two essential transcription activator genes (xyr1 and ace3) increased in response to Zn²⁺ stimulation. Transcriptome analysis revealed that

SUBMITTER: Li N 

PROVIDER: S-EPMC10148476 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature