Tissue-engineered anterior segment eye cultures demonstrate hallmarks of conventional organ culture.

ABSTRACT:

Background

Glaucoma is a blinding disease largely caused by dysregulation of outflow through the trabecular meshwork (TM), resulting in elevated intraocular pressure (IOP). We hypothesized that transplanting TM cells into a decellularized, tissue-engineered anterior segment eye culture could restore the outflow structure and function.

Methods

Porcine eyes were decellularized with freeze-thaw cycles and perfusion of surfactant. We seeded control scaffolds with CrFK cells transduced with lentiviral vectors to stably express eGFP and compared them to scaffolds seeded with primary TM cells as well as to normal, unaltered eyes. We tracked the repopulation behavior, performed IOP maintenance challenges, and analyzed the histology.

Results

Transplanted cells localized to the

SUBMITTER: Waxman S 

PROVIDER: S-EPMC10148776 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature