MTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolin.

ABSTRACT: mTOR complex 2 (mTORC2) has been implicated as a key regulator of glioblastoma cell migration. However, the roles of mTORC2 in the migrational control process have not been entirely elucidated. Here, we elaborate that active mTORC2 is crucial for GBM cell motility. Inhibition of mTORC2 impaired cell movement and negatively affected microfilament and microtubule functions. We also aimed to characterize important players involved in the regulation of cell migration and other mTORC2-mediated cellular processes in GBM cells. Therefore, we quantitatively characterized the alteration of the mTORC2 interactome under selective conditions using affinity purification-mass spectrometry in glioblastoma. We demonstrated that changes in cell migration ability specifically altered mTORC2-associated prote

SUBMITTER: Chantaravisoot N 

PROVIDER: S-EPMC10148843 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature