Development of two protocols for Agrobacterium-mediated transformation of white clover (Trifolium repens) via the callus system.

ABSTRACT: White clover (Trifolium repens) is one of the most widely cultivated livestock forage plants whose persistence is severely affected by abiotic stresses. For the white clover, efficient regeneration systems is still a great necessity. In this study, inoculating 4-day-old cotyledons into MS media fortified with 0.4 mg·L^-1 6-BA and 2 mg·L^-1 2,4-D significantly increased the callus induction rate. Roots and cotyledons proved to be better explants, followed by hypocotyls, leaves, and petioles for callus induction. The development of differentiated structures occurred effectively on MS supplemented with 1 mg·L^-1 6-BA and 0.1 mg·L^-1 NAA. To increase transformation, we investigated various factors affecting the Agrobacterium tumefaciens transforma

SUBMITTER: Jia T 

PROVIDER: S-EPMC10148932 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature