Metabolic engineering of Bacillus subtilis toward the efficient and stable production of C₃₀-carotenoids.

ABSTRACT: Commercial carotenoid production is dominated by chemical synthesis and plant extraction, both of which are unsustainable and can be detrimental to the environment. A promising alternative for the mass production of carotenoids from both an ecological and commercial perspective is microbial synthesis. To date, C₃₀ carotenoid production in Bacillus subtilis has been achieved using plasmid systems for the overexpression of biosynthetic enzymes. In the present study, we employed a clustered regularly interspaced short palindromic repeat-Cas9 (CRISPR-Cas9) system to develop an efficient, safe, and stable C₃₀ carotenoid-producing B. subtilis strain, devoid of plasmids and antibiotic selection markers. To this end, the expression levels of crtM (dehydrosqualene synthase) an

SUBMITTER: Filluelo O 

PROVIDER: S-EPMC10148934 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature