Regulation of extracellular matrix composition by fibroblasts during perinatal cardiac maturation.

ABSTRACT:

Background

Cardiac fibroblasts are the main non-myocyte population responsible for extracellular matrix (ECM) production. During perinatal development, fibroblast expansion coincides with the transition from hyperplastic to hypertrophic myocardial growth. Therefore, we investigated the consequences of fibroblast loss at the time of cardiomyocyte maturation by depleting fibroblasts in the perinatal mouse.

Methods and results

We evaluated the microenvironment of the perinatal heart in the absence of fibroblasts and the potential functional impact of fibroblast loss in regulation of cardiomyocyte cell cycle arrest and binucleation. Cre-mediated expression of diphtheria toxin A in PDGFRα expressing cells immediately after birth eliminated 70-80% of the cardiac fibroblasts. At po

SUBMITTER: Kuwabara JT 

PROVIDER: S-EPMC10149041 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature